Time Resolved Spectroscopy with ICCD cameras
Intensified cameras enhance the time-resolved Spectroscopy for FLIM, FRET, TIRF or Thomson scattering applications.
The combination of the intensified CCD (ICCD) camera with an spectrometer provides researcher with a unique tool for Time-Resolved Spectroscopy (TRS). Researcher of dynamic processes in any kind of materials, chemical components or biomedical tissues using time-resolving spectrometer to analysis the changing energy distribution of emitted light over time. Although, spectroscopy is one of the oldest and most venerated scientific instrumentation, the combination with a ultra high speed ICCD camera provides a cutting edge instrumentation which enables breakthroughs in many scientific fields.
Cutting edge time-resolved Spectroscopy
Time-resolved spectroscopy is defined as anything that allows you to measure the temporal dynamics and kinetics of photo-physical processes by means of spectroscopic techniques. As an example, the measurement of fluorescence emission of a given material is decaying over time. Most often the researched sample is excited, most commonly by a laser pulse. The resulting emission and their decay times are then measured as a function of time. The high shutter speed of the ICCD camera, with gating times down to 200ps, allow measurements with sub-nanosecond time resolution.
Applications of time-resolved Spectroscopy
Time-resolved spectroscopy has established a solid foothold in chemistry, bio-medicine, metrology and many other scientific fields. It enables incredible accurate measurements of dynamic processes in the sub-nanosecond regime, e.g. the verification of the exponential decay law describing luminescence processes of dissolved organic materials. Increasing interest in time-resolved spectroscopy and time-resolved imaging is coming from biologists. Many biological processes are important to understand and these are often revealed by looking at photons or other emissions as a function of time. The TIR technique e.g. enables the intraoperative differentiation of brain tumor in humans.
Fluorescence lifetime measurements
Another important application is the fluorescence lifetime measurements. With the temporal characteristics of the emitted photons, the fluorescence processes can be modeled, understood and monitored. Therefore the time-resolving spectrometer are applied in Fluorescence Lifetime Imaging Microscopy (FLIM), Fluorescence Resonance Energy Transfer ( FRET), Total Internal Reflection Fluorescence (TIRF) or Sub-ns Thomson scattering.